LOCUS       Exported                3053 bp DNA     circular SYN 14-JUL-2025
DEFINITION  Expression vector pTD1(TM) DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Suzuki T, Ito M, Ezure T, Kobayashi S, Shikata M, Tanimizu K, 
            Nishimura O.
  TITLE     Performance of expression vector, pTD1, in insect cell-free 
            translation system
  JOURNAL   J. Biosci. Bioeng. 102 (1), 69-71 (2006)
  PUBMED    16952840
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Suzuki T, Shikata M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2004) Masamitsu Shikata, SHIMADZU CORPORATION, 
            Analytical & Measuring Instruments Division, Life Science 
            Laboratory; 1, Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto, Kyoto 
            604-8511, Japan (E-mail:mshikata@shimadzu.co.jp, 
            URL:http://www.shimadzu.com, Tel:81-75-823-1351, Fax:81-75-823-1368)
REFERENCE   3  (bases 1 to 3053)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3053)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3053)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biosci.
            Bioeng."; date: "2006"; volume: "102"; issue: "1"; pages: "69-71"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-NOV-2004) Masamitsu Shikata, SHIMADZU CORPORATION, Analytical ";
            pages: "81-75-823-1368"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        240..258
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           259..304
                     /label=polyhedrin 5' UTR
                     /note="5' UTR from the Malacosoma neustria
                     nucleopolyhedrovirus polyhedrin gene (Ezure et al., 2006)"
     misc_feature    305..342
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     3'UTR           359..731
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     misc_feature    736..757
                     /label=synthetic poly(A)20 region
                     /note="synthetic poly(A)20 region"
     terminator      759..806
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(832..848)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(856..872)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(880..910)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(925..946)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1234..1822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1996..2853)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2854..2958)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcctt
      241 aatacgactc actataggag tatttttatt ctttcgtaaa aaaattagaa aaataaaata
      301 taaagatatc gaattcgagc tcggtacccg gggatcctct agagtcgggc ggctgtaaaa
      361 cacgatacat tgttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac
      421 agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt
      481 tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc
      541 aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat
      601 ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag
      661 caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt
      721 caaaatatta tccgcaaaaa aaaaaaaaaa aaaaaaaact agcataaccc cttggggcct
      781 ctaaacgggt cttgaggggt tttttgcggc cgcaagcttg gcgtaatcat ggtcatagct
      841 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      901 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      961 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1021 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1081 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1141 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1201 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1261 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1321 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1381 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1441 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1501 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1561 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1621 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1681 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1741 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1801 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1861 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1921 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1981 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2041 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2101 accatctggc cccagtgctg caatgatacc gcgagatcca cgctcaccgg ctccagattt
     2161 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2221 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2281 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2341 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2401 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2461 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2521 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2581 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2641 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2701 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2761 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2821 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2881 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2941 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     3001 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
